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. 2015 Mar 5;6(11):9031–9044. doi: 10.18632/oncotarget.3320

Figure 3. WIP1 modulates cell migration and invasion by regulating MMP-9 and VEGF-C expression.

Figure 3

(A), Western blotting analysis of WIP1-pcDNA co-cultured with WIP1-shRNA1 ACC-M cells. WIP1 silencing and overexpression were determined by Western blotting. ß-actin loading control is also shown. Representative of three independent experiments was shown. (B and C), The quantitative analysis of migration (B) and invasion (C) in WIP1-pcDNA co-cultured with WIP1 -shRNA1 ACC-M cells. Representative images of migrated and invaded cells were shown under inverted microscopy. The mean was derived from cell counts of 5 fields, and each experiment was repeated 3 times. (D), Western blotting analysis of MMP-9, VEGF-C, MMP-2, MMP-13, and VEGF-D in WIP1-pcDNA co-cultured with WIP1-shRNA1 ACC-M cells. ß-actin loading control is also shown. Representative of three independent experiments was shown. (E), Transcription levels of MMP-9, VEGF-C, MMP-2, MMP-13, and VEGF-D in WIP1-pcDNA co-cultured with WIP1 -shRNA1 ACC-M cells, relative to GAPDH, determined by quantitative RT-PCR. Error bars represent the mean ± SD of triplicate experiments (*p < 0.05).