Figure 4. Lifetime monitoring of RMS in heterozygous Ptch mice after exposure to 5-aza-dC.

Pregnant mice were injected with 5-aza-dC at 9.5, 10.5 and 11.5 dpc and formation of palpable RMS in the Ptch^+/− offspring (dashed line) was monitored weekly until the age of 250 days. Ptch^+/− control animals (continuous line) were not exposed to 5-aza-dC. Two censored animals are marked by a bar. The difference between both groups is statistically significant (P = 0.0012, Gehan-Breslow-Wilcoxon test).