Figure 1. Growth behavior and morphology changes of SLC25A10 knockdown cells.

Establishment of stable SLC25A10 knockdown cell lines with specific siRNA expression in A549 cell line. (A) SLC25A10 gene expression was analysed by real-time qPCR in different clones (siRNA-SLC-2, siRNA-SLC-4 and siRNA-SLC-5). (B) SLC25A10 protein expression in A549 cells; 1, untransfected cells; 2, siRNA-CON cells; 3, siRNA-SLC-2; 4, siRNA-SLC-5. (C) Growth curve of siRNA-SLC-2 cell line, data is represented as mean ± SD. (D) Cell morphology change (magnification 20X) and mitochondrial staining. Cells with altered mitochondria are indicated with arrows and scale bar represents 20 μm (E) Sizes of colonies. (F) Numbers of colonies. This experiment was repeated 3 times independently, data is presented as mean ± SD, *** represents p < 0, 001. (G) Mitochondrial DRP1 and MFN1 gene expression levels.