Figure 1. Inducible CIP4 silencing and EGFR activation in TNBC cells alters EGFR signaling.

(A) MDA-MB-231 cells transduced with Tripz (vector) or a Dox-inducible shRNA targeting CIP4 (shCIP4) were treated with the indicated doses of Dox (μg/ml) for 48 hours. Lysates were subjected to immunoblot with the indicated antibodies. (B) MDA-MB-231 Tripz or shCIP4 were treated with Dox (2 μg/ml for 48 hours) prior to serum starvation and treatment with EGF (50 ng/ml) for the indicated times (min.). Lysates were subjected to immunoblot with the indicated antibodies. Densitometry was performed and phosphoprotein levels were normalized to total protein levels, and expressed as fold change relative to time 0. Positions of molecular mass markers are indicated on the left.