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. 2015 Mar 19;6(11):9397–9408. doi: 10.18632/oncotarget.3351

Figure 2. CIP4 silencing alters EGFR signaling to Akt and ERK kinases.

Figure 2

(A) MDA-MB-231 Tripz or shCIP4 were treated with Dox (2 μg/ml for 48 hours) prior to serum starvation and treatment with EGF (50 ng/ml) for the indicated times (min.). Lysates were subjected to immunoblot with the indicated antibodies. Positions of molecular mass markers are indicated on the left. (B) Densitometry was performed and phosphoprotein levels were normalized to total protein levels, and expressed as fold change relative to time 0 (mean ± sem is shown for 4 separate blots from 2 experiments; *p < 0.05, **p < 0.01).