Skip to main content
. 2015 Mar 19;6(11):9409–9419. doi: 10.18632/oncotarget.3352

Figure 2. NF-κB dual-regulates HIF-1α under short-term versus prolonged hypoxia.

Figure 2

(A) Vista predictions of NF-κB binding sites on the promoter of HIF1A. (B) ChIP analysis of the HIF1A promoter with NF-κB subunit antibodies and control IgG. (C,D) After HepG2 cells were transfected with shNC, shp65 or shcRel, and exposed to hypoxia for 4 h (H4) or 24 h (H24), the mRNA (C) and protein (D) levels of HIF-1α were assessed. The results were expressed as the mean±SEM of four independent experiments. *P < 0.05 and **P < 0.01 compared with shNC plus H4; #P < 0.05 compared with shNC plus H24. (E,F) After HepG2 cells were transfected with mock, p65 or cRel, and exposed to hypoxia for 4 h (H4) or 24 h (H24), the mRNA (C) and protein (D) levels of HIF-1α were assessed. The results were expressed as the mean±SEM of four independent experiments. *P < 0.05 and **P < 0.01 compared with mock plus H4; #P < 0.05 compared with mock plus H24.