Figure 5. Dicer1 contributes to HIF-1α degradation under prolonged hypoxia.

After HepG2 cells were transfected with shNC or shDicer1, and exposed to hypoxia for 0 h (N), 4 h (H4) or 24 h (H24), the mRNA (A) and protein (B) levels of HIF-1α were assessed. The results were expressed as the mean±SEM of four independent experiments. **P < 0.01 compared with shNC plus N; ^&P < 0.05 and ^&&P < 0.01 compared with shNC plus H4; ^##P < 0.01 compared with shNC plus H24.