Figure 4. Reduction of Fyn or Yes expression increases Claudin-2 levels while reduced Lyn expression lowers Claudin-2 levels in breast cancer cells.

Immunoblot analysis of Claudin-2 expression in human breast cancer cells (MDA-MB-231 and BRC31) harbouring a control vector (LucA) or infected with two independent shRNA expression vectors (KD1 and KD2) against c-Src (A), Fyn (B), Yes (C) or Lyn (D). As a loading control, total cell lysates were blotted for α-Tubulin.