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. 2015 Mar 25;6(11):9502–9516. doi: 10.18632/oncotarget.3321

Figure 2. Knockdown of TRAIL-R2 reverses the bone-metastatic signature of MDA-MB-231-BO cells and impairs proliferation and migration.

Figure 2

TRAIL-R2 was either transiently (siRNA) or stably (shRNA) down regulated in MDA-MB-231-BO cells. Protein levels were determined by Western blotting in whole cell lysates (A). β-actin levels were used as a loading control. (B) Proliferation of cells with either transient (siRNA) or stable (shRNA) down regulation of TRAIL-R2 was determined by cell counting 72 h post transfection or 72 h after seeding, respectively, and graphed relative to their individual controls (n = 3). (C) TRAIL-R2 knockdown cells (R2-shRNA) or control cells (Ctrl-shRNA) were analyzed in regard to their migration capacity towards FCS in trans-well assays. Graphs represent average values ± SD (n = 4) (*p < 0.05, **p < 0.01, ***p < 0.001).