Fig. 5.

Relative bioluminescence activity and emission spectra of living HEK293T cells expressing mammalian codon-optimized PLG2 (black) and luc2 (green). (A) Bioluminescence was initiated by the addition of 0.1 ml of 10 mM LH₂ to wells containing equivalent numbers of cells in 0.1 ml of media and then monitored (triplicate assays) at 37 °C. (B) Normalized bioluminescence spectra of living cells at 37 °C. The spectra were collected 1 min after the addition of 10 mM LH₂ (0.25 ml) to a cuvette containing 0.25 ml of cells at 37 °C.