Figure 5. STAT6- or mitochondrial ROS-independent inhibition of NLRP3 inflammasome by IL-4.

(a) Murine BMMs from wild-type (WT) or Stat6-deficient mice were untreated (Unt) or primed with LPS (0.25 μg ml⁻¹, 3h) in the presence or absence of IL-4 pretreatment (20 ng ml⁻¹, 3h or 18h) followed with ATP (2.5 mM, 30 min). Soluble lysates were immunoblotted as indicated. (b) Culture supernatants from (a) were assayed for extracellular IL-1β or IL-6 by ELISA. (c) RNA extracts from (a) were assayed for mRNA production of arginase1 (Arg1) by RT-PCR. (d) Murine BMMs were untreated (Unt) or treated with LPS (0.25 μg ml⁻¹, 3h) in the presence or absence of IL-4 pretreatment (20 ng ml⁻¹, 3h) followed with ATP stimulation (2.5 mM, 30 min). Cells were stained with MitoSox and analyzed by flow cytometry.