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. Author manuscript; available in PMC: 2016 Aug 7.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Jun 4;463(4):667–672. doi: 10.1016/j.bbrc.2015.05.122

Figure 2.

Figure 2

IL-1β did not affect cell proliferation and stimulated lactate production with alterations of glucose metabolic enzymes in inTPCs. A, The inTPCs were treated with the indicated concentrations of IL-1β for 7 days and subjected to CYQUANT cell proliferation assay. B, Pictures of inTPC cultures treated in absence of presence of 5 ng/ml IL-1β and/or 1 mM DCA for 3 days. C, The media of inTPCs cultures were collected after treatment with vehicle (Control) or 5 ng/ml IL-1β for 6, 24, 48 or 72 hours and subjected to lactate assay. *, p<0.05 Control to IL-1β at 48 and 72 h; **, p<0.05 DCA group to either Control or IL-β+DCA group at 48 and 72 h. D, The inTPC cultures were treated with vehicle (Control) or 5 ng/ml IL-1β and subjected to Immunoblot analysis for hekisokinase II (HKII), lactate dehydrogenase (LDHA), pyruvate dehydrogenase (PDHA) or α-tubulin (α-Tub). E, The inTPCs were treated with or without IL-1β (5 ng/ml) and DCA (1mM) for 7 days and subjected to qPCR for gene expression of Collagen 1 and Scleraxis. Values are average and SD for 4 samples. *, p< 0.05 to Control.