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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2015 May 12;14(7):1717–1727. doi: 10.1158/1535-7163.MCT-14-0607

Figure 3. W2476T CD24+ cells preferentially expresses stem cell genes and have increased levels of pSTAT3.

Figure 3

(A) qRT-PCR Comparison of stem cell gene expression in W2476T whole cell line and W2476T sorted CD24+ and CD24− cells. (B) Western blot showing differential phosphorylation of STAT3 in CD24+ and CD24− W2476T cells and inhibition by Stattic and TG101209. (C) Percent viable W2476T cells treated with increasing doses of TG101209. (Di) Tumor sphere formation of W2476T cells treated with Stattic and TG101209 for three consecutive days and counted on day 5. (Dii) Passage and formation of secondary spheres from spheres grown in Di. Results show preferential inhibition of spheres formed from CD24+ W2476T cells. (Diii) Percentage of CD24+ W2476T cells after treatment with increasing doses of TG101209. * indicates p≤0.05, ** indicates p≤0.01.