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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Med. 2015 Jun 15;21(7):769–776. doi: 10.1038/nm.3872

Figure 2.

Figure 2

Ucn3–null islets are deficient in delta cell number and somatostatin secretion. Transcriptome analysis of Ucn3–null islets compared to wild–type littermates for Ucn3 (a) Sst (b), Hhex (c), and Rbp4 (d) (n = 3), data normalized to 1×107 reads. (e) Somatostatin, insulin and glucagon content of Ucn3–null and control islets (n = 4). (f) Relative delta, beta and alpha cell number of Ucn3 null and control islets (n = 3 control/4 Ucn3 null, 5–7 islets/animal). (g) Somatostatin secretion from Ucn3–null islets compared to control (n = 4, 95 islets/well, asterisks indicate significance with the 5.5 mM glucose group for the same genotype, unless indicated otherwise). Insulin secretion from Ucn3–null islets compared to control in static incubation (h) (n = 4, 7 islets/well) and in islet perfusion (i) (n = 2, 150 islets per chamber, representative of 2 experiments). Significance determined by Student’s t–test (e, f), two–way ANOVA for treatment and genotype, followed by Holm–Sidak’s multiple comparison test (g, h), or two–way ANOVA for genotype and its interaction with time for each block (i); P values for genotype are given (i). All values are mean ± s.e.m., *P < 0.05, **P < 0.01, ***P < 0.001.