Figure 2. NM IIB ablation cell-autonomously disrupts the pathway-specific development of synaptic excitation in CA1 neurons.

(A) The illustration shows the design of these experiments with electrode replacement either in proximal (C, I) or in distal (F) stimulation locations in separate sets of experiments. (B) Representative DIC and fluorescent images of Myh10^fl/fl hippocampus show sparse Cre expression. Representative AMPA (inward, recorded at −70 mV) and NMDA currents (outward, recorded at +40 mV) from (C, I) Myh10^fl/fl and (F) Myh10+/+ animals injected with Cre. Arrows point to where AMPA and NMDA measurements were made. Recordings from neighboring Cre negative and Cre positive neurons (Myh10 KO) show (D) reduced AMPA (paired t(20)=2.33, p=0.03, n=21 pairs from n=9 mice ) and (E) reduced NMDA currents (paired t(14)=2.16, p=0.048, n=15 pairs from n=7 mice) at proximal stimulation locations, but (G) normal AMPA (paired t(17)=0.99, p=0.33, n=18 pairs from n=3 mice) and (H) increased NMDA currents (paired t(15)=2.57, p=0.021, n=16 pairs from n=3 mice) at distal stimulation locations. (I-K) Control experiments at proximal locations show that Cre positive neurons (wt/td) have (G) normal AMPA (paired t(15)=0.50, p=0.63, n=16 pairs from n=5 mice) and (H) normal NMDA currents (paired t(11)=0.11, p=0.92, n=12 pairs from n=5 mice) from Myh10^+/+ animals. Dashed black line is identity line. Filled circle is the sample mean. Error bars represent SEM.