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. 2015 Mar 12;6(12):10016–10029. doi: 10.18632/oncotarget.3540

Figure 1. Stat3 activation in breast cancer brain metastases and WP1066 inhibited brain metastasis.

Figure 1

(A) pStat3, Stat3, and β-actin protein expression levels in MDA-MB-231 and BT-474 cells. (B) Expression levels of pStat3 in 90 IDC and 89 breast cancer brain metastasis specimens. ***, P<0.001. (C) MDA-MB-231BR cells and BT-474BR cells were treated with 1 μM WP1066 (wp) for the indicated times, and whole-cell lysates were subjected to western blotting for pStat3 (Tyr705), Stat3 and β-actin. (D) Concentration of WP1066 in mouse brain tissue and plasma after WP1066 treatment for 72 hours. (E) The effect of WP1066 on the brain metastases of MDA-MB231-BR cell in vivo. 5×105 MDA-M-B231-BR cells were injected into the Left ventricle of the heart of nude mice. Results were shown for one representative experiment of two. **, P<0.01, ***, P<0.001. (F) HE-stained sections of brain metastases of MDA-MB-231BR cells in mice. (G) Survival of mice injected with MDA-MB-231BR cells and given later WP1066 treatment. Data are presented from the day of injection to day 100. Survival of mice was evaluated by Kaplan-Meier analysis. ***, P<0.001.