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. 2015 Mar 12;6(12):10045–10059. doi: 10.18632/oncotarget.3541

Figure 2. VASN is highly expressed in HCC patient serum, tissues and cell lines.

Figure 2

(a) VASN was verified to be high in HCC sera by a quantitative ELISA assay. The results confirmed the elevation of circulating VASN of HCC patients compared to that of control cohorts. (b) The ROC curve was generated and the area under the curve (AUC) is 0.770. (c) Among the 37 cases of AFP negative serum (37%), 62% of samples were VASN positive (≥ 1.5061ng/ml). (d) VASN mRNA was more highly expressed in hepatocarcinoma tissues than those in hepatocirrhosis, hepatitis and pericarcinoma tissues. (e) VASN mRNA was highly expressed in human hepatoma HepG2, SMMC-7721 cells as measured by real-time PCR. (f) The protein level of VASN was relatively high in human hepatoma HepG2, SMMC-7721 cells measured by Western blot. (g) The expression and localization of VASN protein were verified on HCC tissue slides and benign lesion (hepatitis) liver tissue slides by an indirect immunofluorescence assay. (h) VASN was located on the hepatocellular carcinoma cell surface according to immunofluorescence staining.