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. 2015 Mar 12;6(12):10045–10059. doi: 10.18632/oncotarget.3541

Figure 3. VASN increases hepatoma cell proliferation and migration and inhibited cell apoptosis.

Figure 3

(a) The knockdown effect of siRNA targeting VASN was confirmed at the mRNA level by q-PCR and (b) at the protein level by Western blot and (c) by immunofluorescence in HepG2 cells. (d) Knockdown of VASN in HepG2 cells resulted in decreased cell proliferation. (e) Knockdown of VASN in HepG2 cells resulted in increased apoptosis. (f) Knockdown of VASN in HepG2 cells resulted in reduced migration. (g) Over-expression of full-length GFP-tagged VASN in L02 cells was confirmed by fluorescence microscopy,and (h) Western blot. (i) Overexpression of full-length GFP-tagged VASN in normal L02 cells resulted in increased cell proliferation measured by the MTS assay, which VASN-specific siRNA can abolish. (j) Transwell chamber migration assay confirmed that overexpressed VASN allows increased cell migration in L02 cells.