Skip to main content
. 2015 Mar 12;6(12):10045–10059. doi: 10.18632/oncotarget.3541

Figure 6. Forced Overexpression of miR146a downregulated cell growth and migration and increased cell apoptosis through downregulation of VASN expression in HepG2 cells.

Figure 6

(a) The expression level of VASN mRNA was down regulated after transient transfection of miR146a mimics into HepG2 cells by real-time PCR. (b) The expression level of VASN protein was down regulated after transient transfection of miR146a mimics into HepG2 cells by Western blot. (c) Overexpression of miR146a inhibited HepG2 cell growth by MTS assay. (d) Overexpression of miR146a inhibited HepG2 cell migration by transwell chamber migration assay. (e) Overexpression of miR 146a mimics in HepG2 inhibited migration in a scratch wound assay. Upper: A scratch was created with a 200 μl-pipette tip and photographed. Lower: Photographs at 36h after scratching. (f) Overexpression of miR146a induced HepG2 cell apoptosis.