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. 2015 Mar 12;6(12):10102–10115. doi: 10.18632/oncotarget.3546

Figure 7. Immunohistochemical patterns of PIK3CA and Yap in a mixed human hepatocellular carcinoma (HCC)/cholangiocarcinoma (CCA).

Figure 7

Upper panel: the tumor exhibits areas with strong immunoreactivity for Hep Par1 (a hepatocellular marker, stained in red) intermingled with other areas positive for CK7 (a cholangiocellular marker, stained in brown) immunohistochemistry. Middle panel: Area of the mixed human HCC/CCA with mainly hepatocellular differentiation (as indicated by the large prevalence of Hep Par1 positive cells over those displaying CK7 immunoreactivity) shows strong immunolabeling for both PIK3CA and Yap proteins, with the latter mainly localized in the nucleus of malignant cells. Lower panel: Area of the same tumor with predominant cholangiocellular featues (as demonstrated by the strong CK7 staining) exhibiting a homogeneous and pronounced immunoreactivity for PIK3CA and Yap proteins. Abbreviations: CCA, cholangiocarcinoma; HCC, hepatocellular carcinoma; HE, hematoxylin and eosin staining. Original magnification: 20X in upper panel; 200X in middle and lower panel.