Figure 5. Blockage of ICD generation by γ-secretase inhibitor and fate of ICD.

(A) After overnight treatment with compound E, a γ-secretase inhibitor, the cells were treated with YD alone or co-treated with MG132 for 3 h. The collected cell lysates were analyzed by western blot with an antibody against C-terminal AXL using β-actin as a loading control. (B) The cells were treated with YD alone or co-treated with MG132 for 3 h. The lysates were immunoprecipitated with anti-C-terminal AXL and immunoblotted using anti-ubiquitin (Ub). AXL western blotting was performed on the total lysates. The β-actin immunoblotting of the total lysates is shown to normalize the input. The results are representative of three independent experiments.