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. 2015 Mar 19;6(12):10498–10509. doi: 10.18632/oncotarget.3403

Figure 5. Cbl/Cbl-b DKO HSCs show impaired in vivo reconstitution ability.

Figure 5

(A) HSC limiting-dilution transplantation. Indicated numbers of LSKs were transplanted and mice with < 0.5% donor-derived chimerism at 16 weeks were considered non-responders. Data from two experiments are pooled. (B) 2000 LSKs were transplanted and donor-cell chimerism in PB analyzed at the indicated times. Data from two experiments are pooled. (C–D) Donor LSK pool was analyzed in recipient BM at around 20 weeks. Representative FACS plot (C) and quantitation (D) are shown only for recipients with > 70% donor cell reconstitution. Data from three experiments are pooled. (E–F) Secondary transplants with 2000 donor LSKs sorted from primary recipients transplanted 20 weeks earlier. Donor cell chimerism in PB (G) and WBC counts (H) over time are shown. Data from two repeats are pooled (*p < 0.05).