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. 2015 Mar 25;6(12):10563–10576. doi: 10.18632/oncotarget.3384

Figure 4. iVR1 inhibited the recruitment of smooth muscle cells in xenograft colorectal tumors blocking VEGFR1 activation.

Figure 4

(A) Smooth muscle α-actin (SMA)-positive vessels were counted on five optical fields for each tumor to quantify density of vessels covered by SMCs. *p < 0.020 and §p < 0.05 versus vehicle and CP. (B) Representative pictures of SMA staining (brown) of HCT-116 tumors. Scale bar, 100 μm. (C) Top, western blot analysis of VEGFR1 phosphorylation performed on mixed matching amounts of protein extracts belonging to the same experimental tumor group. Low, normalization with anti-VEGFR1 antibody performed on the same filter. The values of densitometry analyses are shown. Values (in percentages) were calculated as the ratio of degree of receptor phosphorylation with respect to the total receptor amounts. The value of 100 has been arbitrarily assigned to vehicle.