Table 1. Quantification of human Alu sequences in DNA extracted from mice lungs.

Standard curve		Sample analysis
Human genomic DNA [ng]	CTm ± SD	Sample	CTm ± SD	ng
200	19.02 ± 0.45	Vehicle	19.99 ± 0.20	121.87 ± 1.22
20	22.67 ± 0.83	CP	19.70 ± 0.36	145.91 ± 2.67
2	26.37 ± 0.52	bevaciz	22.68 ± 0.04	22.93* ± 0.04
0.2	29.75 ± 085	iVR1	30.05 ± 0.87	0.24* ± 0.01
0.02	33.99 ± 1.03

Standard concentrations of human genomic DNA were used as template for qRT-PCR to amplify Alu sequences. For each concentration analyzed, the average values of cycle threshold (CTm) ± SD are reported (each point in triplicate) and a standard curve was constructed. Genomic DNA extracted from lungs of mice treated with vehicle, iVR1, CP and bevacizumab (bevaciz) (N = 5 per group) were amplified (see Supplementary Figure 1). Based on the average value of cycle threshold (CTm) obtained, the quantity of human Alu sequences were extrapolated by comparison with the standard curve, using exponential regression.

^*p < 0.0001 versus vehicle and CP.