Skip to main content
. 2015 Apr 14;6(12):9701–9717. doi: 10.18632/oncotarget.3832

Figure 4. Inhibiting autophagy with ATG5 siRNA reduced the increase in autophagy caused by EGCG.

Figure 4

ATG5 small interfering RNA (siATG5) or negative control siRNA (NC) transfected primary neuron cells were incubated with 50 μM PrP (106-126) for 36 h in the presence of EGCG. Cell viability was measured by annexin V assay A, B. siATG5 or NC transfected primary neuron cells were incubated with EGCG (10 μM) for 30 h. Western blot for ATG5, LC3 and p62 proteins was analyzed from SH-SY5Y cells. β-actin was used as loading control C. siATG5 or NC transfected SH-SY5Y cells were incubated with 50 μM PrP (106-126) for 36 hr in the presence of EGCG. Cell viability was measured by Annexin V assay D, E. siATG5 or NC transfected SH-SY5Y cells were incubated with EGCG (10 μM) for 30 hr. Western blot for ATG5, LC3-II and p62 proteins was analyzed from SH-SY5Y cells. Beta-actin was used as the loading control F. Bars indicate mean ± standard error (n = 4). *p < 0.05, **p < 0.01, significant differences between control and each treatment group, and #p < 0.01; significantly different when compared with PrP (106-126)-treated group.