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. 2015 Apr 7;6(12):9781–9793. doi: 10.18632/oncotarget.3382

Figure 5. Silencing Flot2 alone does not changed NPC cell motility.

Figure 5

(A) Efficiency of three siRNA sequences against Flot2 was evaluated by Quantitative real-time PCR. (B to D) CNE-1 cell motility was not affected by any of the three Flot2-silencing siRNAs. (B) Cell scattering and morphology. Scale bar = 50μm. (C) Wound-healing assay and (D) Transwell assay. (E) Flot2 silencing has no influence on the expression of EMT makers (E-cadherin, vimentin and β-catenin), TGF-β receptor 1 or total Src in CNE-1 cells. #P<0.01, +P<0.001.