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. 2015 Mar 18;6(12):9794–9806. doi: 10.18632/oncotarget.3400

Figure 1. The growth-suppressive effect of Ntn4 knockdown on GC cells.

Figure 1

A–B. Ntn4 was efficiently knockdown by two independent Ntn4 siRNAs in AGS and MGC803 cells. Ntn4 expression was examined by Q-PCR (A) and ELISA (B) after transfection for 48 hours. C–D. Ntn4 ablation suppressed the proliferation of gastric cancer cells by CCK8 assays. The siRNAs were transfected into AGS (C) and MGC803 (D) cells as indicated for 24–72 hours. E. Ntn4 silencing reduced colony formation in AGS and MGC803 cells. Data are presented as mean ± SEM from three independent experiments with each running in triplicate. Unpaired student's t-test was used for the comparison between the two groups (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).