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. 2015 Mar 18;6(12):9794–9806. doi: 10.18632/oncotarget.3400

Figure 2. Ntn4 silencing suppressed the migration and invasion of GC cells.

Figure 2

A–B. Ntn4 knockdown slowed the wound healing in AGS and MGC803 cells. The monolayer of cells transfected with siControl or siNtn4-1/2 was disrupted with a tip, the cells migrated into the disrupted gap of the monolayer were observed under microscope and photographed at 0, 15, 24, and 48 hours; Magnification, × 100 (A); the gap size was measured and plotted as the percentage of the original time point (0 hour) (B). C. Ntn4 knockdown interrupted cell invasion. siControl or siNtn4 cells were plated into a matrigel-coated 8 μm tranwell. After 24 hours incubation, invaded cells on the bottom surface were fixed and counted after staining with crystal violet; Magnification, × 100. Mean ± SEM, n ≥ 3. **p < 0.01, ***p < 0.001.