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. 2015 Mar 18;6(12):9794–9806. doi: 10.18632/oncotarget.3400

Figure 3. Overexpression of Ntn4 promoted the growth, migration and invasion of GC cells.

Figure 3

A–B. The overexpression of Ntn4 was achieved in AGS and MGC cells by transient transfection of pcDNA3-Ntn4 for 48 hours and with pcDNA3 vector (pcDNA3) only as control. Expression of Ntn4 was measured with Q-PCR (A) and ELISA (B). C–D. The enhanced expression of Ntn4 accelerated the growth of AGS (C) and MGC803 (D) cells. The cell proliferation assay was performed with CCK8 assay at 24, 48 and 72 hours, respectively after the transfection with pcDNA3 or pcDNA3-Ntn4. E. The enhanced expression of Ntn4 boosted the wound healing of AGS and MGC803 cells. The monolayer of cells transfected with pcDNA3 or pcDNA3-Ntn4 was disrupted with a tip and photographed under microscope at 0 and 24 hours; Magnification, × 100. F–G. The enhanced expression of Ntn4 promoted the invasion of AGS (F) and MGC803 (G) cells. Cells were transfected with pcDNA3 or pcDNA3-Ntn4 for 48 hours and subjected to the invasion assay; Magnification, × 100. Mean ± SEM, n ≥ 3. **p < 0.01.