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. 2015 Mar 18;6(12):9794–9806. doi: 10.18632/oncotarget.3400

Figure 5. Neo knockdown reversed the promoted effects of Ntn4 on cell proliferation and invasion.

Figure 5

A–B. Ntn4 overexpression promoted cell growth in a Neo dependent manner. The cell proliferation was performed in AGS (A) and MGC803 (B) cells co-transfected with pcDNA3+siC, pcDNA3+siNeo, pcDNA3-Ntn4+siC or pcDNA3-Ntn4+siNeo for 48 hours with CCK8 assay. C–E. Ntn4 overexpression couldn't increase cell invasion in the absence of Neo. Cells were co-transfected with pcDNA3+siC, pcDNA3+siNeo, pcDNA3-Ntn4+siC or pcDNA3-Ntn4+siNeo for 48 hours and subjected to the invasion assay (C); Magnification, × 100. The invaded AGS (D) or MGC803 (E) cells on the bottom surface were counted and analyzed. Mean ± SEM, n ≥ 3. **p < 0.01, ***p < 0.001.