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. 2015 Mar 21;6(12):9877–9886. doi: 10.18632/oncotarget.3185

Figure 3. Phosphorylation of MORC2 at Ser-677 promotes gastric cell proliferation and cell cycle progression.

Figure 3

(A and B) MORC2 Phosphorylation promotes gastric cell proliferation. (A) The stable expressing lentivirus-mediated Flag-MORC2 SGC-7901 and MGC-803 cells grown for 2,4 or 6 days and analyzed for cell growth by cell counts assay. Values are the means ± SD from three individual experiments. (B) Colony formation assays were performed with the lentivirus-mediated stable expressing of Flag-MORC2 SGC-7901 and MGC-803 cells. Representative results are shown. (C–D) Phosphorylation of MORC2 promotes cells transition from G1 to S. (C) The lentivirus-mediated stable expressing of Flag-MORC2 of SGC-7901 and MGC-803 cells were cultured and stained with propidum iodide. Cell cycle distribution was measured by FACS. Values are the means ± SD from three individual experiments. The average percents of three experiments were showed in histogram. (D) The levels of these proteins controlling cell cycle were detected by Western blotting in the lentivirus-mediated stable expressing of Flag-MORC2 SGC-7901 and MGC-803 cells. The lysates were probed with indicated antibodies.