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. 2015 Mar 26;6(12):9970–9984. doi: 10.18632/oncotarget.3667

Figure 3. TRAIL and delphinidin induces apoptosis via activation of caspases in LNCaP cells.

Figure 3

(A and B) LNCaP cells were treated with TRAIL (TR, 50 ng/ml) and/or delphinidin (DEL, 30 μM) for 12 h. (A) The cell morphology was visualized by an inverted microscope (magnification ×100). (B) By DAPI staining, the cells clearly show condensed chromatin or fragmented nuclei which were taken as apoptotic bodies (magnification ×1000). (C and D) TRAIL-mediated apoptosis with delphinidin was suppressed by caspase inhibitor zVAD. LNCaP cells were pretreated for 30 min with or without zVAD (40 μM) before TRAIL (50 ng/ml) and/or delphinidin (30 μM) treatment for 12 h. The increased apoptosis from combination treatment was blocked by zVAD (C) and the levels of caspase-8, caspase-9, and cleaved caspase-3 and caspase-7 were reduced by zVAD treatment on western blot analysis (D). (E-G) TRAIL and delphinidin dramatically induced activation of caspases in LNCaP cells. LNCaP cells were pretreated for 30 min with or without zVAD (40 μM) before TRAIL (50 ng/ml) and/or delphinidin (30 μM) treatment for 12 h. After incubation, the activities of caspase-3/7, caspase-8, and caspase-9 were inhibited by zVAD treatment. All data are expressed as the mean ± SD for triplicates (*P<0.05 vs. −TRAIL/−DEL; **P<0.05 vs. with +TRAIL/+DEL).