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. 2014 Sep 22;12(4):444–455. doi: 10.1038/cmi.2014.70

Figure 4.

Figure 4

Cytotoxicity of PBMCs toward MSCs and alloantibody production in the serum at various time points post-transplantation. PBMCs collected from recipients of each group on days 1, 7, 14 and 28 post-transplantation were considered as effector cells, and then cocultured with MSCs (target cell to effector cell ratio: 1∶10) for 72 h. PBMCs from the TVT group (Normal+PBS) as control. Percentage of apoptotic MSCs were evaluated by annexin V-APC/7-AAD staining and flow cytometry (a). **P<0.01. Peripheral blood serum was collected from recipients on days 7, 14 and 28 post-transplantation, and then incubated with MSCs. Incubated samples were stained with a RPE-conjugated secondary antibody against rat IgG1. Micrographs illustrate the IgG1 antibody in the MSC-treated pancreas group on days 14 and 28 (b). MSC: mesenchymal stem cell; PBMC: peripheral blood mononuclear cell; TVT: tail vein transplantation.