Figure 4.

Spontaneous CII reactivity in MZ B cells but not in B-1 B cells. Splenic B cells from naïve and BCII-immunized mice (n = 4–6) were separated into B-1 and MZ B cells by FACS. (a) The total cell count of splenic B-1 B cells and MZ B cells in naïve and BCII-immunized mice. (b) IgM anti-BCII antibodies in culture supernatants of B-1 B cells and MZ B cells stimulated with CpG. Data were presented as mean + SEM and represent three independent experiments. OD = optical density; dpi = days post-immunization.