FIG 7.

Growth/survival of hvKP1ΔiucA (aerobactin deficient) in human ascites fluid with and without the addition of enterobactin, salmochelin, yersiniabactin, and aerobactin. The growth/survival of hvKP1ΔiucA, was assessed by measurement of CFU at 0, 3, 6, and 24 h in 90% human ascites fluid–10% 1× PBS (pH 7.4) with or without the addition of purified siderophores at the concentrations of 0 nM, 40 nM, 80 nM, 160 nM, 320 nM, 640 nM and 1.28 μM. The listed order of concentrations in each panel is based on plateau density. Data are means ± SEM; four to six independent growth curves were performed for each strain at each siderophore concentration. The growth/survival of hvKP1ΔiucA was significantly increased with the addition of 80 nM, 160 nM, 320 nM, 640 nM, and 1.28 μM enterobactin, with the addition of 1.28 μM yersiniabactin, and with 640 nM and 1.28 μM aerobactin compared to growth in the absence of exogenous siderophore (*, P < 0.05/6, two-tailed unpaired t test).