FIG 2.

Increased Tim-3 expression was associated with increased concentrations of schistosome antigens. Splenic immune cells from 6-week-old male C57BL/6 mice were collected and stimulated with 0, 10, 20, and 50 μg/ml SWA or SEA, respectively. After 24 h of incubation, cells were collected. (A) Representative histograms of Tim-3 expression on CD3⁺ cells detected by flow cytometry. The plots are representative of three independent experiments with five to seven mice in each group per experiment. (B) Comparisons of the relative expression of Tim-3 in the CD3⁺ cells under indicated antigen concentrations (means ± SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001).