FIG 4.

Increased expression of Tim-3 induced by SWA and SEA led to NK cell loss. Splenic immune cells from wild-type C57BL/6 mice were treated with SWA and SEA. (A and B) Cells were collected at 0, 24, and 48 h and analyzed by flow cytometry. (A) Representative histograms of Tim-3 expression on CD3⁻ NK1.1⁺ cells. (B) Representative histograms of CD3⁻ NK1.1⁺ cell proportions in lymphocytes. (C) Cells were collected after 48 h of incubation, and NK cells were isolated using magnetic beads. The apoptosis of NK cells was detected by flow cytometry. The results are representative of two independent experiments.