FIG 5.

The regulatory transcriptome of R. equi 103S^P− transformed with virR, virS, and vapA closely resembles the wild-type R. equi regulatory transcriptome. Bacteria were grown to an optical density at 600 nm of 1 under vapA-inducing growth conditions (LB at 37°C and pH 5.5). RNA isolation, cDNA synthesis, and labeling and microarray hybridization were performed for four biological replicates as described in Materials and Methods. Shown is a heat map of differentially expressed genes annotated as regulators. Above the heat map is a Euclidian distance-based dendrogram of strains based on changed regulators. Microarray data were analyzed with ArrayStar (DNAStar).