FIG 6.

TLR2, but not TLR4, mediates macrophage inhibition of C. muridarum at intermediate MOIs. (A) Macrophages pretreated with anti-TLR2 were infected at an MOI of 0.5 (black bars) or 10 (gray bars). IFN-β levels in supernatants at 24 h p.i. were measured by ELISA. (B) IFU from macrophages infected with C. muridarum at an MOI of 0.5 at 24 h p.i. Some wells exposed to E. coli LPS were treated with anti-TLR4 or IgG2A isotype control Ab (10 μg/ml). (C and D) IFU at 24 h p.i. from macrophages infected at an MOI of 10 treated with anti-TLR2 or IgG1 isotype control Ab (30 μg/ml) (C) and anti-TLR4 or IgG2A (D). ****, P < 0.0001; ***, P < 0.001. Shown are means and SD.