FIG 7.

Moderately infected RAW macrophages inhibit C. muridarum by producing nitric oxide and reactive oxygen species. (A) Supernatants and protein were collected at 6, 12, or 24 h p.i. from RAW cells infected at an MOI of 0.5 (black bars) or 10 (gray bars). Nitrite levels were quantified by Griess assay. (B) Western blotting for iNOS levels. (C) RAW cells infected at an MOI of 0.5 (black bars), 10 (gray bars), or 10 with DMTU (white bars) were assayed for ROS production at 1, 3, 6, 12, 18, and 24 h p.i. (D) RAW cells infected at an MOI of 0.5 or 10 were left untreated (black bars) or were treated with l-NMMA (white bars), l-NAME (gray bars), or DMTU (hatched bars). IFU assays were performed 24 h p.i. ****, P < 0.0001; **, P < 0.01; *, P < 0.05. Shown are means and SD.