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The classical complement pathway mediates C3 binding to P. aeruginosa. Mid-log-phase cultures of wild-type PAO1 and oprF-deficient P. aeruginosa were incubated in 20% C1q-depleted human serum, C1q-repleted serum or heat-inactivated serum for 20 min at 37°C. The bacterial pellets were washed, and C3 binding was analyzed by flow cytometry. Means ± SEM are given (n = 3). **, P ≤ 0.005; ***, P ≤ 0.0005.
