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Subcellular localization of five PvTRAg proteins in asexual blood-stage parasites of P. vivax. The 4% paraformaldehyde-fixed ring-stage (A) and trophozoite/schizont-stage (B) P. vivax parasites were dually labeled with a mouse anti-PvPHIST/CVC-8195 polyclonal antibody (green; caveola-vesicle complex marker) and a rabbit immune serum against each PvTRAg protein (red). DIC, differential interference contrast; Merge, merged images of PvTRAgs and PvPHIST/CVC-8195. Scale bars, 5 μm.
