TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Vibrio vulnificus strains | ||
| CMCP6 | Clinical isolate | 54 |
| YJ016 | Clinical isolate | 55 |
| JBL0808 (Δnab2) | CMCP6 Δnab2 (VV1_0808) | This study |
| JBL0808 (Δnab2) Smr | CMCP6 Δnab2; spontaneously Sm-resistant isolate | This study |
| JBL0808C | CMCP6 Δnab2/pnab2 (pBB0808c) | This study |
| JBL0808C Smr | CMCP6 Δnab2/pnab2 (pBB0808c); spontaneously Sm-resistant isolate | This study |
| SAM0312 (Δnab2) | YJ016 Δnab2 (VV0312) | This study |
| SAM0312 (Δnab2) Smr | YJ016 Δnab2 (VV0312); Sm-resistant isolate | This study |
| JBL1950 (ΔflhF) Smr | CMCP6 ΔflhF (VV1_1950); Sm-resistant isolate | This study |
| JBL1950 (ΔflhF) | CMCP6 ΔflhF (VV1_1950) | This study |
| Escherichia coli strains | ||
| DH5α λ-pir | pir ϕ80dlacZΔM15 δ(lacZYA-argF)U169 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1 | |
| β2155 DAP | Donor for bacterial conjugation; thr-1004 pro thi strA hsdS lacZΔM15 (F lacZΔM15 lacTRQJ36 proAB) dapA Ermr pirRP4 (Kmr from SM10) | |
| Plasmids | ||
| pJet1.2/blunt | High-copy-number cloning vector | |
| pJet10808SOE | V. vulnificus VV1_0808 (nab2) SOE product | This study |
| pJet0312SOE | V. vulnificus VV0312 (nab1) SOE product | This study |
| pJet1950SOE | V. vulnificus VV1_1950 (flhF) SOE product | This study |
| pDS132 | Suicide plasmid; Cmr sacB | 30 |
| pDS0808 | V. vulnificus nab2 knockout vector (VV1_0808 SOE product) | This study |
| pDS0312 | V. vulnificus nab1 knockout vector (VV0312 SOE product) | This study |
| pDS1950 | V. vulnificus flhF knockout vector (VV1_1950 SOE product) | This study |
| pBBR1MCS | Expression plasmid; empty vector; Cmr | 56 |
| pBB0808c | nab2 (VV1_0808) expression plasmid (pnab2) | This study |