FIG 6.

lplA1-deficient L. monocytogenes had a severe dissemination defect in mice. BALB mice were coinfected with a 1:1 mixture of L. monocytogenes SD2301 (ΔlplA1) and the complemented mutant L. monocytogenes SD2302 (+lplA1) for a total inoculum of 3 × 10⁸ to 7 × 10⁸ CFU. Pooled data from at least two separate experiments are shown. (A) The percentage of each strain recovered from the MLN 3 days postinfection is expressed as a competitive index (CI); the solid horizontal line indicates the geometric mean. (B and C) Absolute number of CFU of each strain recovered 3 (B) or 4 (C) days postinfection. Solid horizontal lines indicate mean values, and dashed lines indicate limits of detection.43