TABLE 1.
Replication of F. tularensis strains and L. monocytogenes upon phagocytic uptake or microinjection into various cell types
| Bacterium | Growth in cell line afterd: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Phagocytosisa |
Microinjectionb |
|||||||||
| J774 | BMDM | ASC−/− | MyD88−/− | HeLa | J774 | BMDM | ASC−/− | MyD88−/− | HeLa | |
| Listeria monocytogenes | +++c | +++c | +++c | +++c | +++c | +++ | +++ | +++ | − | +++ |
| F. tularensis strains | ||||||||||
| LVS | ++ | ++ | ++ | +++ | ++ | ++ | ± | ++ | ± | +++ |
| ΔiglA mutant | − | − | − | − | − | ++ | ++ | +++ | +++ | ++ |
| ΔiglC mutant | − | − | − | − | − | ++ | + | +++ | +++ | +++ |
| ΔiglG mutant | +++ | ++ | ++ | + | +++ | +++ | ++ | +++ | ++ | +++ |
| ΔiglI mutant | +++ | − | + | + | ++ | ++ | ++ | +++ | +++ | +++ |
| ΔpdpE mutant | +++ | +++ | +++ | +++ | +++ | +++ | ++ | +++ | +++ | +++ |
a
The actual numbers of bacteria are shown in Fig. S1 to S6 in the supplemental material.
b
The estimated number of bacteria per cell and the differences indicated are based on statistical evaluations as summarized in Table 2.
c
Due to rapid growth, the number of L. monocytogenes bacteria was determined at 8 h.
d
Bacterial numbers were recorded after 24 h and are represented as a sliding scale ranging from very significant replication (+++) to no replication (−).