TABLE 1.
B. burgdorferi Δglp-infected nymphs transmit infection to naive mice
| Strain | Description | No. of bite sites with infected nymphs/no. of bite sites analyzeda,b | No. of infected mice/total no. of mice tested 4 wk after drop-off byb,c: |
|
|---|---|---|---|---|
| Serology | Ear biopsy | |||
| Bb1399 | WT 5A4 NP1 parent | 18/18 | 3/3 | 3/3 |
| Bb1197 | 5A4 NP1 Δhk1 | 0/16d | 0/3 | 0/3 |
| Bb1451 | 5A4 NP1 Δrrp1 | 0/12 | 0/3 | 0/3 |
| Bb1452 | 5A4 NP1 Δglp | 9/18 | 2/3 | 2/3 |
a
The skin of C3H/HeJ mice (6 sites per mouse) was excised from the site at ∼48 to 72 h postrepletion. A minimum of 2 mice were tested per isolate per time point.
b
Cultures were monitored for spirochetes by dark-field microscopy for at least 4 weeks.
c
Serology and ear biopsies were performed at 4 weeks postrepletion. Ear tissues were cultured in BSK-II medium containing the appropriate antibiotics.
d
Two cultures were discarded due to contamination.