TABLE 4.
Quantitative proteomic profiling of surface-associated proteins in PB3 persistent bacteremia isolates and resolved bacteremia (RB) EMRSA-15 isolates compared to the PB3 initial isolatea
| Accession no. | Protein name | Gene | 0.5 log2 ratio |
|||
|---|---|---|---|---|---|---|
| PB3-32-1 | PB3-74 | RB1 | RB5 | |||
| Q5HEI2 | Map adherence protein | eap | 1.1 | −0.20 | 0.0 | −0.3 |
| Q2FIK4 | Extracelllar matrix binding protein | emp | 1.4 | −0.15 | −0.2 | −0.8 |
| Q2YVZ4 | Immunoglobin binding protein | sbi | 0.7 | −0.10 | 0.55 | −0.95 |
| Q2FH36 | Cold shock protein | cspA | 0.75 | 0.10 | −0.4 | −0.55 |
| Q5HJW3 | Penicillin binding protein 2 | pbp2′ | 1.1 | 0.05 | −0.1 | −1.15 |
| A7WZT2 | Enolase | eno | 0.1 | −0.15 | −0.70 | −0.5 |
| A7X1J8 | Acyl carrier protein | 0.8 | 0.00 | 0.0 | −0.25 | |
| Q2YUM5 | RNA polymerase | rpoE | 0.95 | 0.30 | 0.1 | 0.35 |
| Q2FHT6 | Thioredoxin | trx | 1.1 | 0.00 | −0.45 | −0.45 |
Bold values represent significant relative changes of 0.5 log2 ratio differences between the initial PB1-1 isolate and persistent PB1 infection isolates or resolved EMRSA-15 infection isolates. Values without a minus sign represent increases; values with a minus sign represent decreases. Relative protein change data represent averages of the results of two biological repeats (inclusive of two technical repeats). Proteins identified showed P values of <0.001 and <0.0001, calculated using the Scaffold Q+ proteomic software from individual ANOVA tests, showing the significance of the difference in protein abundance between two conditions.