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. 2015 May 12;5(2):197–213. doi: 10.3390/membranes5020197

Figure 1.

Figure 1

CCh stimulation induces internalization of M2-EGFP and M2-DsRed in HEK 293T cells. HEK 293T cells were transiently transfected with plasmids coding for M2-EGFP or M2-DsRed. The cells were starved for 18 h in serum-free medium and either left untreated or stimulated with 1 mM CCh for 15 min. Cells were fixed and the nuclei stained with DAPI (shown in blue). Scale bars: 10 μm.