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. 2015 Apr 16;5(2):485–504. doi: 10.3390/biom5020485

Figure 6.

Figure 6

Quantitative analysis of PTPIP51 and RelA and PTPIP51 and IkBα interactions evaluated by Duolink Image Tool software. (A) PTPIP51 and RelA interactions in human keratinocytes. Untreated controls, 50 ng/mL TNFα treated cells, 100 ng/mL TNFα treated cells, 200 ng/mL TNFα treated cells, 400 ng/mL TNFα treated cells, 500 ng/mL TNFα treated cells. * (p < 0.05), ** (p < 0.01); (B) PTPIP51 and IkBα interactions. Left panel: Untreated controls, 50 ng/mL TNFα treated cells, 100 ng/mL TNFα treated cells, 200 ng/mL TNFα treated cells, 400 ng/mL TNFα treated cells, 500 ng/mL TNFα treated cells. *** (p < 0.001). Right panel: controls, 50 ng/mL TNFα treated cells, 100 ng/mL TNFα treated cells, 200 ng/mL TNFα treated cells, 400 ng/mL TNFα treated cells, 500 ng/mL TNFα treated cells. All cells were treated with 50 µM PDTC. The resulting data were analyzed by GraphPad Prism 6 software, significance of results tested by Dunnett’s multiple comparisons test.