Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 30;5(2):679–701. doi: 10.3390/biom5020679

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The tight interaction of BR with protein leads to underestimation of enzyme activities using the classical HO-assay. (A) Relationship between input and extractable amount of BR from HO-assay buffer (amount of BR was calculated using OD at 450 nm corrected for background OD at 520 nm (Diff. OD)) was linear (no protein added); (B) Presence of protein (liver homogenate: HOMO) in assay buffer supplemented with BR (1 µM) decreased the extractable amount of BR (Diff. OD); (C) Relationship between input and extractable amount of BR (0.01–1 µM) from HO-assay buffer (Diff. OD) was linear at constant protein concentration (added tissue homogenate (HOMO) with constant protein concentration of 10 mg protein/mL); (D) Using the polynomial for correcting the BR amount, the activity of HO (formation of BR/30 min) depended nearly linearly on the amount of liver homogenate used for the assay.